高效液相色谱法测定肝复康中芍药苷的含量
孙立华, 胡永明, 马建华
(青岛药品检验所, 青岛 266071 )
[摘要] 目的:建立高效液相色谱法测定肝复康中芍药苷的含量。方法:使用Kromsail C18柱(5μm,250mm×4.6mm) ,流动相:甲醇-水 (35:65);紫外检测波长:230nm。结果:本法测定芍药苷的线性范围为35~176μg·mL-1 (r= 0.9998),平均回收率为99.86%,RSD为0.77%(n=6)。结论:本法操作简单,快速,准确,可行。
[关键词] 芍药苷;高效液相色谱法;肝复康
[中图分类号] R284.1 [文献标识码] B [文章编号] 1003-3734(2001)-200554
HPLC determination of paeoniflorinin in Ganfukang gramda
SUN Li-hua , HU Yong-ming , MA Jian-hua
(Qingdao Institute For Drug Control , Qingdao 266071,China)
[Abstract] Objective: To establish method for determination of paeoniflorinin in Ganfukang gramda. Methods: A Kromasil C18 column(250mm×4.6mm ,5μm ),an UV detector at 230nm and a mobile phase composed of MeOH:H2O=35:65 were adopted in the analysis. Results: The linear range of paeoniflorinin was 35~176μg·mL-1 (r=0.9998). The recovery of paeoniflorinin was 99.86% with a RSD of 0.77%(n=6) . Conclusions: A simple, rapid, accurate and reliable method for determination of paeoniflorinin in ganfukang gramda was established .
[Key Words] paeoniflorinin; HPLC ; ganfukang gramda
[作者简介] 孙立华 (1963-),女,副主任药师,主要从事中(成)药分析及其质量标准的研究工作。联系电话:(0532)5735184。
[ 参考文献 ]
[1] 中华人民共和国国家药典委员会.中华人民共和国药典[S]2000年版. 北京.化学工业出版社,2000: 一部 78-79.
[2] 王宝琴. 中成药质量标准与标准物质研究[M]. 北京:中国医药科技出版社,1994. 32-43.
[3] 梁宏 , 王春芳. 高效液相色谱法测定八珍丸中芍药苷的含量[J]. 药物分析杂志,1999,9(1):44-45.
[4] 金芳, 姚仲青, 顾家栋. 大孔吸附树脂预分离在高效液相色谱法测定芍药苷中的应用[J]. 中国中药杂志,1999,24(10):608-609., 百拇医药
(青岛药品检验所, 青岛 266071 )
[摘要] 目的:建立高效液相色谱法测定肝复康中芍药苷的含量。方法:使用Kromsail C18柱(5μm,250mm×4.6mm) ,流动相:甲醇-水 (35:65);紫外检测波长:230nm。结果:本法测定芍药苷的线性范围为35~176μg·mL-1 (r= 0.9998),平均回收率为99.86%,RSD为0.77%(n=6)。结论:本法操作简单,快速,准确,可行。
[关键词] 芍药苷;高效液相色谱法;肝复康
[中图分类号] R284.1 [文献标识码] B [文章编号] 1003-3734(2001)-200554
HPLC determination of paeoniflorinin in Ganfukang gramda
SUN Li-hua , HU Yong-ming , MA Jian-hua
(Qingdao Institute For Drug Control , Qingdao 266071,China)
[Abstract] Objective: To establish method for determination of paeoniflorinin in Ganfukang gramda. Methods: A Kromasil C18 column(250mm×4.6mm ,5μm ),an UV detector at 230nm and a mobile phase composed of MeOH:H2O=35:65 were adopted in the analysis. Results: The linear range of paeoniflorinin was 35~176μg·mL-1 (r=0.9998). The recovery of paeoniflorinin was 99.86% with a RSD of 0.77%(n=6) . Conclusions: A simple, rapid, accurate and reliable method for determination of paeoniflorinin in ganfukang gramda was established .
[Key Words] paeoniflorinin; HPLC ; ganfukang gramda
[作者简介] 孙立华 (1963-),女,副主任药师,主要从事中(成)药分析及其质量标准的研究工作。联系电话:(0532)5735184。
[ 参考文献 ]
[1] 中华人民共和国国家药典委员会.中华人民共和国药典[S]2000年版. 北京.化学工业出版社,2000: 一部 78-79.
[2] 王宝琴. 中成药质量标准与标准物质研究[M]. 北京:中国医药科技出版社,1994. 32-43.
[3] 梁宏 , 王春芳. 高效液相色谱法测定八珍丸中芍药苷的含量[J]. 药物分析杂志,1999,9(1):44-45.
[4] 金芳, 姚仲青, 顾家栋. 大孔吸附树脂预分离在高效液相色谱法测定芍药苷中的应用[J]. 中国中药杂志,1999,24(10):608-609., 百拇医药