外源性VEGF联合 “夹心法”矫治半侧颜面短小的实验研究(1)
[摘要]目的：探索血管内皮生长因子、纤维蛋白胶及珊瑚人工骨复合物結合下颌骨外板劈开即“夹心法”成骨过程中组织形态学变化，为进一步的实验研究及以后的临床应用提供理论依据。方法：以小型猪为实验对象，A组左侧行下颌骨外板劈开，颗粒状珊瑚羟基磷灰石、血管内皮生长因子及纤维蛋白胶(CHA+VEGF+FS)复合物植入术，右侧作为正常对照组。B组左侧行下颌骨外板劈开，颗粒状珊瑚羟基磷灰石、血管内皮生长因子及纤维蛋白胶(CHA+VEGF+FS)复合物植入术；右侧行下颌骨外板劈开，颗粒状珊瑚羟基磷灰石及纤维蛋白胶(CHA+FS)复合物植入术。通过组织形态学观察等手段研究VEGF对新骨形成过程的影响，评估术后效果。结果：研究发现CHA+VEGF+FS复合物植入后可以促进珊瑚人工骨实现早期血管化，从而减低骨再生过程中珊瑚人工骨的无效降解。结论：CHA+VEGF+FS夹心法可以为珊瑚人工骨提供更好的成骨条件，从而更好实现骨再生，最终使得其可以作为矫治半侧颜面短小导致的下颌骨发育不良畸形的有效手段之一。
[关键词]下颌骨外板劈开术；珊瑚人工骨；半侧颜面短小；血管内皮生长因子；纤维蛋白胶
, http://www.100md.com
[中图分类号]R782.2 [文献标志码]A [文章编号]1008-6455(2018)09-0117-04
The Foundation Research of “Sandwich” Method Combined with Exogenous VEGF for the Treatment of Hemifacial Microsomia
XU Jia，LI Xi，QIAO Jia，LIU Jian-feng，NIU Feng，GUI Lai
(The 6th Department of Plastic Surgery Hospital，Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing 100144，China)
, 百拇医药 Abstract： Objective To discuss the morphological changes in the process of vascular endothelial growth factor and fibrin glue and coral artificial bone complex combined with the osteotomy of outer mandibular cortex，in order to carry out a further study and provide theoretical basis for clinical application in the future. Methods 10 miniature pigs were selected as the experimental animals and then were divided into 2 groups randomly： group A and group B. In group A， the outer cortex of mandibular body and angle was split in one side and the compound of CHA+VEGF+FS was implanted into the gap between the outer and inner cortex of mandible， and the other side was control group. In group B， two kinds of compounds (the left side： the compound of CHA+VEGF+FS， the right side： the compound of CHA+FS ) was implanted into the gaps with the same methods separately.The osteogenesis of this method was assessed by the morphological， histological observation. Results We found that CHA+VEGF+FS complex can promote the vascularization of coral artificial bone earlier， along with reducing the ineffective degradation of coral artificial bone during the process of bone regeneration. Conclusion The results of this pilot study indicate that the osteotomy of the outer mandibular cortex combined with the compound of CHA+VEGF+FS can provide a sufficient osteogenesis for the artificial coral bone during the process of bone regeneration， and finally， it can be used as one of the effective methods to correct the dysplasia of the mandible caused by hemifacial microsomia.
Key words： osteotomy of the outer mandibular cortex； coralline hydroxyapatite； hemifacial microsomia； vascular endothelial growth factor； fibrin sealant, http://www.100md.com(徐佳 李茜 乔嘉 刘剑锋 牛峰 归来)
[关键词]下颌骨外板劈开术；珊瑚人工骨；半侧颜面短小；血管内皮生长因子；纤维蛋白胶
, http://www.100md.com
[中图分类号]R782.2 [文献标志码]A [文章编号]1008-6455(2018)09-0117-04
The Foundation Research of “Sandwich” Method Combined with Exogenous VEGF for the Treatment of Hemifacial Microsomia
XU Jia，LI Xi，QIAO Jia，LIU Jian-feng，NIU Feng，GUI Lai
(The 6th Department of Plastic Surgery Hospital，Chinese Academy of Medical Sciences & Peking Union Medical College，Beijing 100144，China)
, 百拇医药 Abstract： Objective To discuss the morphological changes in the process of vascular endothelial growth factor and fibrin glue and coral artificial bone complex combined with the osteotomy of outer mandibular cortex，in order to carry out a further study and provide theoretical basis for clinical application in the future. Methods 10 miniature pigs were selected as the experimental animals and then were divided into 2 groups randomly： group A and group B. In group A， the outer cortex of mandibular body and angle was split in one side and the compound of CHA+VEGF+FS was implanted into the gap between the outer and inner cortex of mandible， and the other side was control group. In group B， two kinds of compounds (the left side： the compound of CHA+VEGF+FS， the right side： the compound of CHA+FS ) was implanted into the gaps with the same methods separately.The osteogenesis of this method was assessed by the morphological， histological observation. Results We found that CHA+VEGF+FS complex can promote the vascularization of coral artificial bone earlier， along with reducing the ineffective degradation of coral artificial bone during the process of bone regeneration. Conclusion The results of this pilot study indicate that the osteotomy of the outer mandibular cortex combined with the compound of CHA+VEGF+FS can provide a sufficient osteogenesis for the artificial coral bone during the process of bone regeneration， and finally， it can be used as one of the effective methods to correct the dysplasia of the mandible caused by hemifacial microsomia.
Key words： osteotomy of the outer mandibular cortex； coralline hydroxyapatite； hemifacial microsomia； vascular endothelial growth factor； fibrin sealant, http://www.100md.com(徐佳 李茜 乔嘉 刘剑锋 牛峰 归来)