葛根素注射液治疗糖尿病性骨质疏松的实验研究(1)
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摘 要：目的：探讨葛根素注射液对糖尿病骨质疏松的保护作用。方法：30只SD大鼠随机分为正常对照组、糖尿病组和糖尿病葛根素治疗组(n=10)。糖尿病组和糖尿病葛根素治疗组大鼠经腹腔注射链脲佐菌素(65mg/kg)制作糖尿病模型，成模后糖尿病葛根素治疗组每天腹腔注射葛根素注射液80mg/kg，正常对照组和糖尿病组注射等量生理盐水。成模6周后麻处死大鼠，分离各组大鼠的左侧股骨，于小动物放射成像系统上，测其近段骨密度，HE染色进行形态学观察。结果：成模6周后，糖尿病组骨密度显著低于对照组，糖尿病大鼠其股骨头表面成骨细胞与对照组比较显著减少，空骨陷窝明显增多，而葛根素治疗组上述指标均明显改善。结论：高糖可影响骨组织代谢及成骨细胞的增殖分化，造成糖尿病骨质疏松症，而葛根素对骨质疏松的进程可起到一定的保护作用。
关键词：糖尿病；骨质疏松；葛根素
中图分类号：R285.6
文献标识码：A
文章编号：1673-7717(2012)04-0848-03
Experiment Study on Protection of Puerarin in Diabetic Osteoporosis
LI Yong-hua，PAN Han-song, LIANG Yi-min
(Taizhou First People's Hospital，Huangyan 318020，Zhejiang，China)
Abstract:Objective：To explore the protective effect of puerarin on diabetic osteoporosis rats. Methods：Thirty male SD rats were divided into three groups，including control group(intraperitoneally injected saline 6 mL/kg)，diabetic group(intraperitoneally injected STZ 65 mg/kg)and puerarin treatment group(intraperitoneally injected STZ 65 mg/kg，and intraperitoneally injected puerarin 80 mg/kg/day for 6 weeks). After 6 weeks，the rats were isolated left femur，the bone mineral density of proximal femur was measured by the small animal x-ray imaging device，and the morphological changes were also observed. Results：Bone mineral density in diabetic group was significantly lower than that in control group. Haematoxylin-eosin(HE)staining of femur revealed the femoral head surface of osteoblasts was a significant reduce and empty lacunae increased. But the osteoporosis performance was significantly improved in the puerarin group rats.Conclusion：High blood sugar can affect bone metabolism and osteoblasts proliferation and differentiation，resulting in diabetes，and the puerarin may play a protective role in the process.
Key words:diabetes；osteoporosis；puerarin
糖尿病性骨质疏松症(Diabetic osteoporosis)是糖尿病(Diabetes mellitus)患者常见的慢性并发症之一[1]。随着糖尿病发生率的逐年增加，糖尿病骨质疏松症的发病率亦明显上升。研究表明糖尿病骨质疏松症患者存在骨量减少，骨密度降低，骨组织结构受损等病理改变，因此易导致病理性骨折，致残率高，给糖尿病患者带来严重的健康损害，并给社会造成很大的负担[2]。有研究表明，雌激素可预防治疗骨质疏松，长久以来，治疗骨质疏松第一线药物为激素替代疗法，然而长期大剂量服用雌激素有很强的不良反应[3]。因此能有效治疗糖尿病骨质疏松症而同时不良反应较小药物的研究正越来越受到各国研究人员的关注。
葛根是豆科植物野葛或甘葛藤的干燥根，其应用范围涉及心血管、神经、内分泌及眼科等各个领域。许多研究表明，葛根中含有多种异黄酮成分，包括葛根素和大豆黄酮等，这些异黄酮成分在体内外均都能改善骨质代谢，对于骨质疏松具有明显的防治作用，临床上亦有关于此的例证[4] ......
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