基于CRISPR—Cas9定向编辑CCL17和CCL22基因的研究(5)
[19]Kang S，Xie J，Ma S，et al.Targeted knock down of CCL22 and CCL17 by siRNA during DC differentiation and maturation affects the recruitment of T subsets[J].Immunobiology，2010，215(2)：153-162.
[20]Curiel TJ，Coukos G，Zou L，et al.Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival[J].Nat Med，2004，10(9)：942-949.
[21]Tani K，Azuma M，Nakazaki Y，et al.Phase I study of autologous tumor vaccines transduced with the GM-CSF gene in four patients with stage Ⅳ renal cell cancer in Japan：clinical and immunological findings[J].Mol Ther，2004，10(4)：799-816.
[22]Christian M，Cermak T，Doyle EL，et al.Targeting DNA double-strand breaks with TAL effector nucleases[J].Genetics，2010，186(2)：757-761.
[23]Bibikova M，Beumer K，Trautman JK，et al.Enhancing gene targeting with designed zinc finger nucleases[J].Science，2003，300(5620)：764-764.
[24]Cho SW，Kim S，Kim JM，et al.Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease[J].Nat Biotechnol，2013，31(3)：230-232.
[25]Li J，Shou J，Guo Y，et al.Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas 9[J].J Mol Cell Biol，2015，7(4)：284-287.
(收稿日期：2018-01-30 本文編辑：祁海文), 百拇医药(高淑慧 瞿创 吴凤麟 沈晗)
[20]Curiel TJ，Coukos G，Zou L，et al.Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival[J].Nat Med，2004，10(9)：942-949.
[21]Tani K，Azuma M，Nakazaki Y，et al.Phase I study of autologous tumor vaccines transduced with the GM-CSF gene in four patients with stage Ⅳ renal cell cancer in Japan：clinical and immunological findings[J].Mol Ther，2004，10(4)：799-816.
[22]Christian M，Cermak T，Doyle EL，et al.Targeting DNA double-strand breaks with TAL effector nucleases[J].Genetics，2010，186(2)：757-761.
[23]Bibikova M，Beumer K，Trautman JK，et al.Enhancing gene targeting with designed zinc finger nucleases[J].Science，2003，300(5620)：764-764.
[24]Cho SW，Kim S，Kim JM，et al.Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease[J].Nat Biotechnol，2013，31(3)：230-232.
[25]Li J，Shou J，Guo Y，et al.Efficient inversions and duplications of mammalian regulatory DNA elements and gene clusters by CRISPR/Cas 9[J].J Mol Cell Biol，2015，7(4)：284-287.
(收稿日期：2018-01-30 本文編辑：祁海文), 百拇医药(高淑慧 瞿创 吴凤麟 沈晗)